Figure 8

Immunolabeling of larvae and early juveniles of Wirenia argentea. Maximum intensity projections of confocal image stacks; apical is up and scale bar equals 40 μm in all panels. A: Labeling of serotonin-like immunoreactive (−LIR) components; 15 to 16 days posthatching (dph) larva scanned in ventral aspect showing lateral (arrows) and ventral (arrowheads) neurite bundles and the ventromedian nerve plexus (double arrowhead); note the clusters of perikarya (asterisks) associated with the ventral neurite bundles. B: Labeling of serotonin-LIR components; 15 to 16 dph larva scanned in dorsal aspect showing lateral (arrows) and ventral (arrowheads) neurite bundles and the ventromedian nerve plexus (double arrowhead); note the ventral commissures associated with the clusters of perikarya (asterisks) on the ventral neurite bundles and the additional connections between the ventral neurite bundles and the ventromedian nerve plexus (x). C: Labeling of serotonin-LIR components; 19 to 21 dph early juvenile stage scanned in ventral aspect showing lateral (arrows) and ventral (arrowheads) neurite bundles and the ventromedian nerve plexus (double arrowhead); note the ventral commissures associated with clusters of perikarya (asterisks) at the ventral neurite bundles and the additional connections between the ventral neurite bundles and the ventromedian nerve plexus (x). D: Labeling of FMRF-amide-LIR components; 19 to 21 dph early juvenile stage scanned in ventral aspect showing lateral (arrows) and ventral (arrowheads) neurite bundles; note the ventral commissures (asterisks) and the fact that the ventromedian nerve plexus is not labeled. Abbreviations: ao, apical organ; cc, cerebral commissure; ip, innervation of pedal pit; lvc, lateroventral connective; pg, pedal ganglion; pnp, prototrochal nerve plexus; pt, prototroch; sna, serotonin-LIR neurites to anterior body region; snt, serotonin-LIR neurites to terminal body region; src, suprarectal commissure; tt, telotroch.