Figure 9

Immunolabeling of a late larva and early juveniles of Gymnomenia pellucida. Maximum intensity projections of confocal image stacks; apical is up and scale bar equals 40 μm in all panels. A: Labeling of serotonin-like immunoreactive (−LIR) components; 15 to 16 days posthatching (dph) larva scanned in ventral aspect showing lateral (arrows) and ventral (arrowheads) neurite bundles and the ventromedian nerve plexus (double arrowhead); note the ventral commissures associated with clusters of perikarya (asterisks) on the ventral neurite bundles and the additional connections between the ventral neurite bundles and the ventromedian nerve plexus (x). B: Labeling of serotonin-LIR components; 19 to 20 dph early juvenile stage scanned in ventral aspect showing lateral (arrows) and ventral (arrowheads) neurite bundles and the ventromedian nerve plexus (double arrowhead); note the ventral commissures associated with clusters of perikarya (asterisks) on the ventral neurite bundles. C: Labeling of serotonin-LIR components; 19 to 20 dph early juvenile stage scanned in left lateral aspect showing lateral (arrows) and ventral (arrowheads) neurite bundles and the ventromedian nerve plexus (double arrowhead); note the ventral commissures associated with clusters of perikarya (asterisks) on the ventral neurite bundles and the additional connections between the ventral neurite bundles and the ventromedian nerve plexus (x). D: Labeling of FMRF-amide-LIR components; 19 to 20 dph early juvenile stage scanned in ventral aspect showing lateral (arrows) and ventral (arrowheads) neurite bundles; note the ventral commissure (asterisk) and the fact that the ventromedian nerve plexus is not labeled. Abbreviations: ao, apical organ; cc, cerebral commissure; ip, innervation of pedal pit; lvc, lateroventral connective; pg, pedal ganglion; pnp, prototrochal nerve plexus; sna, serotonin-LIR neurites to anterior body region; snt, serotonin-LIR neurites to terminal body region; src, suprarectal commissure.