Figure 2

Bony plates and neuromast distribution are correlated through development in completely plated fish. (A-D) Bone development in a single Japanese Pacific (JP) marine fish, repeatedly stained with calcein during development. The standard length of the fish is indicated in mm. The near-vertical white dotted line in (D) indicates the location of the boundary between the Ma and Mp lines. (E-I) Detail of neuromast and plate development along the posterior flank of DASPEI- and calcein-stained JP fish (scale bar = 0.1 mm); (F-I) are from similar portions of the flank as the boxed regions in (A-D), although the pictures are from different fish. The white arrowheads in (I) indicate the location of neuromasts. (J) Compilation of plate presence and neuromast distribution along the length of Manchester Clam Bay (MC) marine fish at a range of sizes, showing the correlation between plate development and neuromast elaboration. Each horizontal row is a single individual fish; fish are sorted in order of total number of plates. Standard length is indicated in mm. The vertical line between columns seven and eight indicates the location of the boundary between the Ma and Mp lines. Green shading indicates the presence of a plate, white indicates no plate, an unfilled box indicates no neuromasts present, a horizontal line indicates neuromasts along the midline, and a vertical box indicates dorso-ventral distribution of neuromasts. DASPEI, 2-(4-(dimethylamino)styryl)-N-ethylpyridinium iodide; JP, Japanese Pacific Ocean marine; Ma, main trunk line, anterior; MC, Manchester Clam Bay marine; Mp, main trunk line, posterior; PB, Paxton benthic.