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Table 1 Tissue and embryo pools of S. officinalis used for cDNA synthesis and subsequent real-time qPCR

From: A new haemocyanin in cuttlefish (Sepia officinalis) eggs: sequence analysis and relevance during ontogeny

Name

Stage (Lemaire)

Tissue

No. of individuals

Input material [mg]

Comment

E1_Y

21-23

Yolk

2

102

No statistics

E1_E1

21-23

Embryo

8

49

 

E1_E2

21-23

Embryo

2

13

 

E2_E1

24-26

Embryo

3

45

 

E2_E2

24-26

Embryo

2

25

 

E2_E3

24-26

Embryo

2

28

 

E3_E1

26-27

Embryo

2

43

 

E3_E2

26-27

Embryo

2

45

 

E3_E3

26-27

Embryo

1

29

 

E3_E4

26-27

Embryo

1

34

 

E3_E5

26-27

Embryo

2

48

 

E3_E6

26-27

Embryo

1

31

 

E4_E1

28-29

Embryo

2

19

 

E4_E2

28-29

Embryo

1

47

 

E4_E3

28-29

Embryo

1

32

 

H_G

30

Gill

4

12

No statistics