Figure 2

Meis2 expression is upregulated in the bat forelimb as compared to the hindlimb. (A) A probe that mapped to a region 5′ of the Meis2 loci (5′-Meis2) showed the highest DE in the microarray analysis, with overexpression in the bat CS17 forelimb (FL) in comparison to the hindlimb (HL) and mouse FL. The probe specific to Meis2 transcripts showed a similar profile of expression but had a lower signal on the array. (B) A schematic diagram indicating the conserved intron/exon structure of Meis2 mRNA transcripts, containing 13 exons (boxed numbers). The regions encoding the Meis domain and homeodomain are indicated. The binding regions of the microarray probes (in green) are shown for 5′-Meis2 and 3′-Meis2. The position of WISH probes corresponding to 5′-Meis2 and 3′-Meis2 are given in blue, while the targets of the reverse transcriptase and quantitative polymerase chain reaction (RT-qPCR) amplification are shown in red. The alternative spliced exons 11′ and 11, and 12a and 12b are shown in red and green, respectively. The asterisk (*) indicates stop codons and the white boxes represent the untranslated regions (UTRs).