Figure 5
From: Wnt affects symmetry and morphogenesis during post-embryonic development in colonial chordates
Ectopic secondary bud formation upon Wnt pathway alteration. B. schlosseri colonies treated with GSK-3β inhibitor (APL) are massively affected in their overall morphology. (A) Ventral views of a colony incubated with Wnt agonists showing ectopic budlets (highlighted with red dashed line) formations from the primary bud (N + 1, 9/8/3). (B) At stage 9/8/1, control colonies of the same genotype treated with DMSO do not show any morphological alteration. (C) Maximum projection of a z-stack of confocal image showing wnt2B expression by FISH. Note the high level of expression of wnt2B within the two ectopic budlets (b) at stage 9/8/3 (N + 1 cycle). The internal vasculature (asterisk) is also labeled with the probe. (D) A sub-clone of the animals in (C) was used as control for the same FISH experiment. The confocal z-stack image shows wnt2B expression located only on the secondary bud (b). Some background staining is visible on the apical side of the primary bud (B), not considered as true fluorescent signal. (E) Measurements of the A/P axes of primary buds at N + 1 cycle within treated colonies (blue) and control colonies (red). BARS: 1 mm (A and B); 200 μm (C and D).