Figure 4

Expression of C. salei atn, otx, six1, and six2 in different stages of development. Embryos are flat-mounted and counter stained with SYBR green. (A1-A3) Cs-atna, (B1-B3) Cs-atnb, (C1-C4) Cs-otxa, (D1-D4) Cs-otxb, (E1-E4) Cs-six1a, (F1-F4) Cs-six1b, (G1-G4) Cs-six3a, (H1-H3) Cs-six3b. (A1), (C1), (D1), (G1) prosomal limb buds; (A2), (B1), (E1), (F1), (G2), (H1) lateral furrow; (C2), (D2), (E2) inversion I; (F3) inversion III; (A3), (B2), (B3), (C3), (D3), (E3), (F3), (G3), (G4), (H2) prosomal shield; (C4), (D4), (E4), (F4), (H3) ventral closure. Thin arrows: secondary eye vesicles, Thick arrows: principal eye anlagen, asterisk: ON of median eyes. ch chelicerae, lb labrum, lf lateral furrow, ls lateral subdivision, ms median subdivision, pc precheliceral region or precheliceral lobes, ps prosomal shield, sto stomodaeum, vs ventral sulcus. Scale bar 200 μm.