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Figure 12 | EvoDevo

Figure 12

From: Par system components are asymmetrically localized in ectodermal epithelia, but not during early development in the sea anemone Nematostella vectensis

Figure 12

In vivo embryonic localization of NvPar-3::mVenus and NvLgl::mCherry during different stages of N. vectensis development. Co-injection of NvPar-3::mVenus (A) and NvLgl::mCherry (B) displays the same distribution observed when we overexpressed each protein separately: co-distribution during earlier stages but asymmetric localization in the cells of later stages (C). Lifeact::mTq2 mRNA (D) was used to visualize the shape of the cells and the apical cortex. NvPar-3::mVenus and NvLgl::mCherry were observed below the cortical actin during all observed stages (E). White arrowheads indicate the localization of NvLgl::mCherry at the cell contacts. All images correspond to a 3D reconstruction from z-stack series. See Additional files 31, 32, 33, and 34 and FigureĀ 13 for more details of every stage presented here.

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