In vivo localization of NvLgl::mCherry during different stages of N. vectensis development. The overexpression of NvLgl::mCherry protein (A) displays similar patterns observed with the antibody staining against the same protein, and no signal was observed in the endoderm. Lifeact::mTq2 mRNA (B) was co-injected to visualize the shape of the cells and the apical cortex. In (C), NvLgl::mCherry is always expressed below the cortical actin and localized in the lateral cortex during all observed stages. White arrows indicate astral/centrosomal and (pro)nuclear structures. White arrowheads indicate the locations adjacent to other cells (cell contacts) where NvLgl::mCherry is localized. Images of unfertilized, cleavage, and side panels in gastrula stages correspond to a single slice from z-stack series. Image of the whole blastula and gastrula corresponds to a 3D reconstruction from z-stack series. Side panels in blastula and gastrula stages are a mid section from the z-stack series to show the basolateral distribution of NvLgl::mCherry along the A/B axis. See Additional files 15, 16, 17, 18, and 19 for more details of every stage presented here in C.