dpp expression and phenotypes generated by parental RNAi. (A-D)
In situ hybridization revealing dpp mRNA expression in early embryos prior to limb bud formation (A), and embryos where the legs are extending (B-D). (E) Untreated embryo developing normally. (F)
dpp RNAi resulted in halting embryonic development at an early stage. dpp: decapentaplegic; WT: wild type; T1, 2, 3: thoracic segments 1, 2, 3.