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Fig. 7 | EvoDevo

Fig. 7

From: Stem cells in Nanomia bijuga (Siphonophora), a colonial animal with localized growth zones

Fig. 7

Assessment of cell proliferation in a mature colony after a five-hour EdU pulse. Nuclei of cells that divided during this interval appear magenta, other nuclei appear blue. a Siphosomal growth zone. b Bright field image of tissue shown in a. Pigment spots cause a red fluorescent signal. c Close-up of region in a showing EdU-labeled nuclei in the horn, young buds, and stem tissue. d Nectosomal growth zone with high densities of EdU-labeled nuclei along the nectosomal stem, in zooid buds and in developing nectophores. e Horn to the left and youngest bud, close-up of box in d. f Siphosomal stem fragment (SF2) with young developing buds, mature palpon, and palpacle base. g Tentacle base and developing tentilla at the base of the gastrozooid basigaster. h Posterior part of the nectosomal stem of the colony shown in d. EdU-labeled nuclei are sparsely scattered (arrowheads). i EdU-labeled nuclei along the stem posterior to the siphosomal growth zone, close-up of box in a. j Posterior siphosomal stem fragment (SF2) with EdU-labeled nuclei absent. k Siphosomal stem fragment (SF1) with EdU-labeled nuclei along the dorsal canal. l–p Ontogenetic series of gastrozooid development. l–n Close-ups of gastrozooids (g) shown in a. o Developing hypostome of a gastrozooid with high densities of EdU-labeled nuclei. p Hypostome of a mature gastrozooid from siphosomal fragment (SF2) with few EdU-labeled nuclei (arrowheads). q Close-up of developing palpon (p) shown in a. r Two mature palpons from siphosomal fragment SF2 with EdU-labeled nuclei absent. s Young developing bract from siphosomal fragment (SF2) with EdU-labeled nuclei. t Mature bract from siphosomal fragment (SF3) with EdU-labeled nuclei absent. u Developing male gonophores from siphosomal fragment SF2. v Mid-section of a late male gonophore from siphosomal fragment SF3. w Developing female gonophores with EdU-labeled cells. a–c, f–g Anterior is up, ventral to the right. d, e Anterior is up, dorsal to the right. h–j Anterior to the left, lateral view. k Anterior to the left, dorsal view. l–w Distal is up. b bract, ba basigaster, db developing bud, dte developing tentilla, dm dorsal midline, fgo female gonophore, g gastrozooid, gc gastric cavity, h horn of the growth zone, mgo male gonophore, ne nectophore, nst nectosomal stem, p palpon, pba palpacle base, pi pigment, sst siphosomal stem, tba tentacle base, * hypostome

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