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Fig. 1 | EvoDevo

Fig. 1

From: Spiralian gastrulation: germ layer formation, morphogenesis, and fate of the blastopore in the slipper snail Crepidula fornicata

Fig. 1

Early epiboly and position of clones at the blastopore lip. a–h Cartoons of early embryo with second and third quartet micromeres colored, as indicated in key to the right. a–c Animal pole views; d is a ventral/vegetal view; e–h are lateral views. Black and white cartoons are modified from Conklin’s drawings [42]. i–r Images of embryos during late cleavage and early epiboly stages labeled with the actin cytoskeleton marker UTPH-GFP (white) and histone H2B-RFP (red); in some panels, the 4d clone is labeled with diI (red). AV animal pole view, VV ventral/vegetal pole view, LV lateral view. The 4d clone can often be identified without direct labeling because the UTPH-GFP is preferentially expressed or stabilized in this clone (e.g. as in l, m, q, r). Scale bar equals 50 μm. s–w Time lapse light sheet confocal images of an embryo expressing the microtubule-cytoskeleton bio-sensor EMTB-GFP and an RFP-membrane biosensor (MEM-RFP). Lateral view. Many cells are seen to divide over the course of the time-lapse (arrow mitotic spindle), but the micromere cap does not make a significant advance towards the vegetal pole during this period. See also Additional file 1, which corresponds to panels s–w

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