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Fig. 11 | EvoDevo

Fig. 11

From: Spiralian gastrulation: germ layer formation, morphogenesis, and fate of the blastopore in the slipper snail Crepidula fornicata

Fig. 11

Fates of micromere 3d, and its subclones, during gastrulation and organogenesis. Images of live embryos, with dextran and diI-labeled 4d, 3d, or 3d subclones, as indicated. In some cases, the zygote was previously injected with mRNAs coding for fluorescent fusion proteins for the actin-binding domain of utrophin-GFP (UTPH) and histone H2B-RFP to visualize nuclei or cell outlines, respectively, where indicated. Animal pole is up in a and b. Anterior is up in c–y. a, b Dorso-lateral views of early epiboly-stage embryos. c, d Ventral views of early epiboly stage embryos. e–j Ventral views of elongating embryos later during epiboly. k, l Corresponding ventral views of an embryo during elongation with different combinations of fluorescence and/or DIC layers shown. m Right-lateral higher magnification views of an elongating embryo with inserts showing some of the ventral ciliated cells (shallow confocal stacks centered at the ventral midline). n, o Corresponding ventral views of embryos during organogenesis. Corresponding left lateral views of embryos during organogenesis p-q, r-s, t-u. Corresponding ventral views of early veliger stage embryos v-w, x-y. ltc left terminal cell. All other labels are the same as those used in Figs. 3, 6, and 10. Scale bar equals 50 μm for a–l and n–y. Scale bar equals 25 μm for m and 20 μm for its inserts

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