Fig. 13

Origin of the anus (2d²). Images of live embryos, with dextran and diI-labeled 4d, 3d, 3c, 2d, or 2d subclones, as indicated. In some cases, the zygote was previously injected with mRNAs coding for fluorescent fusion protein for the actin-binding domain of utrophin-GFP (UTPH) to visualize cell outlines, where indicated. Anterior is up in all cases. a–c Corresponding ventral views of embryos during organogenesis with different combinations of fluorescence and/or DIC layers shown. d, e Corresponding right-lateral (oblique-ventral) views of embryo during organogenesis. f, g Corresponding higher magnification right-lateral views of the posterior end of an embryo during organogenesis. h, i Corresponding ventral views of veliger stage embryo after the anus as opened. j Ventral view of a veliger stage embryo just before the anus opens. Note that the hindgut is somewhat swollen. k, l Corresponding ventral views of pre-veliger stage embryos. m, n Corresponding (oblique-ventral) left-lateral views of the pre-veliger stage embryos. o Ventral view of early veliger. p, q Corresponding (oblique-ventral) left-lateral views of an early veliger. r, s Corresponding ventral views of an early veliger. an anus, nc neural cell, ns neurosensory cell, rm right mantle, tcs terminal cells (called tc in Fig. 6). All other labels are the same as those used in Figs. 3, 4, 10, and 11. Scale bar equals 50 μm for a–e, h–s. Scale bar equals 25 μm in f, g