Fig. 3
Fates of micromere 2a, and its subclones, during gastrulation and organogenesis. Images of live embryos with dextran- and diI-labeled 2a, or 2a subclones, as indicated. In some cases, the zygote was previously injected with mRNAs coding for fluorescent fusion proteins for histone H2B-RFP (H2B) and/or the actin-binding domain of utrophin-GFP (UTPH) to visualize nuclei or cell outlines, respectively, where indicated. Anterior is up in all cases. a Ventral view during early epiboly. Corresponding ventral-view images are shown in b-c, d-e, f-g during late epiboly with different combinations of fluorescence and/or DIC layers shown. h Shows a dorsal view of the same embryo shown in f-g. i, j Ventral views of older elongating embryos. k, l Corresponding right lateral views of an older embryo undergoing organogenesis. m Left lateral view of an older embryo undergoing organogenesis. n, o Corresponding left lateral view of an older embryo undergoing organogenesis. bp blastopore, ft foot, hg hindgut rudiment, mt metatroch, pgc primordial germ cell, rtc right terminal cell, sg shell gland, st stomodeum/mouth. Scale bar equals 50 μm