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Fig. 4 | EvoDevo

Fig. 4

From: Spiralian gastrulation: germ layer formation, morphogenesis, and fate of the blastopore in the slipper snail Crepidula fornicata

Fig. 4

Fates of micromere 2b, and its subclones, during gastrulation and organogenesis. Images of live embryos, with dextran and diI-labeled 2b, or 2b subclones, as indicated. In some cases, the zygote was previously injected with mRNAs coding for fluorescent fusion proteins for histone H2B-RFP (H2B) and/or the actin-binding domain of utrophin-GFP (UTPH) to visualize nuclei or cell outlines, respectively, where indicated. Anterior is up in all cases. a Ventral view during an early stage of epiboly. b Ventral view during a later stage of epiboly. Corresponding ventral views are shown in c-d, e-f, g-h, i-j during epiboly with different combinations of fluorescence and/or DIC layers shown. Corresponding dorsal images are shown in k-l, m-n during epiboly. o–q Ventral views of older, elongated embryos. r Oblique left-lateral view of the ventral surface of an older embryo. Corresponding left-lateral images are depicted in s-t and x-y of older embryos undergoing organogenesis. u Right lateral view of an older embryo undergoing organogenesis. v, w Corresponding oblique left-lateral views of the ventral surface of an older embryo undergoing organogenesis. fg food groove, nc neural cells. Other labels are the same as those used in Fig. 3. Scale bar equals 50 μm

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