Fig. 5

Fates of micromere 2c, and its subclones, during gastrulation and organogenesis. Images of live embryos with dextran and diI labeled 2c, or 2c subclones, as indicated. In some cases, the zygote was previously injected with mRNAs coding for fluorescent fusion proteins for histone H2B-RFP (H2B) and/or the actin-binding domain of utrophin-GFP (UTPH) to visualize nuclei or cell outlines, respectively, where indicated. Anterior is up in all cases. a, b Corresponding ventral and dorsal views of an embryo near the end of gastrulation with different combinations of fluorescence and/or DIC layers shown. c, d Corresponding ventral views of an embryo near the end of gastrulation. e, f Corresponding ventral views of a slightly older embryo at the end of gastrulation. g, h Corresponding ventral views of an older elongating embryo. i Dorsal view of an elongating embryo. j Ventral surface view of an embryo at the onset of organogenesis. k, l Corresponding right lateral views of an embryo at the onset of organogenesis. m, n Corresponding right lateral views of an older embryo during organogenesis. o, p Corresponding oblique dorsal view of an embryo during organogenesis. Corresponding right-lateral views of embryos undergoing organogenesis are shown in q-r, s-t. tc terminal cells. Other labels are the same as those used in Fig. 3. Scale bar equals 50 μm