Fig. 8From: Spiralian gastrulation: germ layer formation, morphogenesis, and fate of the blastopore in the slipper snail Crepidula fornicata Fates of micromere 3b, and its subclones, during gastrulation and organogenesis. Images of live embryos, with dextran and diI-labeled 4d, 3b, or 3b subclones, as indicated. In some cases, the zygote was previously injected with mRNAs coding for fluorescent fusion proteins for histone H2B-RFP (H2B) and/or the actin-binding domain of utrophin-GFP (UTPH) to visualize nuclei or cell outlines, respectively, where indicated. Animal pole is up in a. Anterior is up in b–o. a Lateral-dorsal view of an early epiboly-stage embryo. b-c, d-e show corresponding ventral (vegetal) views of early epiboly-staged embryos with different combinations of fluorescence and/or DIC layers shown. f Ventral view of embryo during later epiboly. g, h Show ventral views of two stages of mesenchyme migration. i Ventral view showing numerous mesenchyme cells. j, k Corresponding right-lateral views of embryos during organogenesis. l, m Ventral surface views. n, o Corresponding right-lateral views of older embryos during organogenesis. Labels are the same as those used in Figs. 3, 6, and 7. Scale bar equals 50 μmBack to article page