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Fig. 9 | EvoDevo

Fig. 9

From: Spiralian gastrulation: germ layer formation, morphogenesis, and fate of the blastopore in the slipper snail Crepidula fornicata

Fig. 9

Behavior of ectomesoderm (3a2, 3b2). a–i Time-lapse movie of an embryo injected with utrophin-GFP (UTPH) to mark cell outlines, and histone H2B-RFP (H2B) to mark nuclei, where indicated. Ventral view. bp blastopore. Several unidentified (x) daughter cells of 3a2, 3b2 are marked, in colors, to show the orientation of cell division and position of ectomesodermal precursor cells during the narrowing of the anterior blastopore lip. Scale bar in f equals 50 μm; scale bar in i equals 25 μm. j–u Frames from a timelapse movie of an embryo injected with ensconsin-GFP (EMTB) to mark microtubules, and histone-RFP (H2B) to mark nuclei. Ventral view. Dashed white lines outline the 3b2 clone. Scale bar in u equals 30 μm. v–z Spinning disk confocal frames from a time-lapse of an embryo expressing utrophin-GFP (UTPH) globally, and in which the 3b micromere was labeled with diI (red). Ventral view. Scale bar in z equals 50 μm. See also Additional files 6, 7, and 8

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