Fig. 5

Quantitation of Dmac-prd pRNAi segmentation defects. Offspring produced by twelve of either Dmac-prd 5′ or 3′ dsRNA injected females, as indicated. Each hatched larva was stretched out using tweezers and examined under a dissecting microscope. a Percent of hatched pRNAi offspring showing segmentation phenotypes. b–c Embryos were collected on the third day after injection and segmentation defects were scored. b Frequency of fusion of specific pairs of adjacent segments. Note that some larvae had more than one pair fused. c Frequency of types of segmentation defects observed. Left bars percentage of hatched larvae with wild-type segmentation, and those displaying one, two, three or four segmental fusions; right bars percentage of hatched larvae with one, two, three or four segmental fusions among those with observable segmentation defects