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Fig. 8 | EvoDevo

Fig. 8

From: Gastrulation occurs in multiple phases at two distinct sites in Latrodectus and Cheiracanthium spiders

Fig. 8

The cumulus internalizes first and its cells have an opisthosomal fate in C. mildei. a Early gastrula soon after injection of CM-DiI (red) into the early deep layer below the blastopore. b Representative vertical section (10 µm, paraffin) through cumulus 6 h after CM-DiI injection. Nuclei stained with DAPI. c First-instar juvenile 10 days after injection, parasagittal section near midline. White arrowheads indicate label. d Heat map showing positions of label in serial sections spanning ~160 µm of the sagittal midline. Counts were summed for four juveniles with a maximum value of 32 points of label counted per grid position. Darker shades of color represent more points per grid position, summed for the four individuals. Additional labeled cells were found in more lateral sections (not shown). Scale bars a, c 200 µm; b 50 µm

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