Fig. 6
Expression of fz1/2/7 during early development in Platynereis. a–i′ WMISH of fz1/2/7, and a′-i″ false color images of the WMISH (red) overlaid with DAPI-stained nuclear images (blue). Animal-pole view (a, a′) and side view with animal pole up (b, b′) of 1 cell embryos. 4-cell (c, c′), 8-cell (d, d′) embryos, animal-pole view. e, e′ 6 hpf embryo, animal-pole view. White arrowheads point to 1q11 cells. 8 hpf embryo animal pole (f, f′) and side (g, g′) views. White arrowheads in f′ point to expression domains in c and d quadrants. White arrowheads in g′ point to expression in c quadrant. 10 hpf embryo animal pole (h, h′) and vegetal pole (i, i′, i″) views. White arrowheads in h′ point to expression in d and a/b quadrants. White arrowheads in i″ point to expression in 2d1121 and 2d1122 progeny. White arrows in i″ point to expression in c, a and b quadrants. Asterisks mark the animal pole. Black arrows indicate the orientation of the dorsal–ventral (D-V) and animal-vegetal (A-V) axis. Black arrowheads in e, f, g, h, and i′ indicate 2d cell lineage. White asterisk in a–h′ indicates location of animal pole