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Fig. 5 | EvoDevo

Fig. 5

From: Posterior eyespots in larval chitons have a molecular identity similar to anterior cerebral eyes in other bilaterians

Fig. 5

Expression of selected apical markers during the development of L.asellus. Column 1–3 single labeling of gene X. Column 4–6 double labeling of gene X (cyan) and Las-r-opsin (magenta) in the anterior, posttrochal eye and posterior region. Las-foxq2 B expression is clearly limited to the apical area in young larvae of 48 hpf (a1) and can be found in later developmental stages in all PRCs (a2a6). Las-otx expression in young larvae of 48 h hpf is limited to the apical region and the prototroch (b1) and can be found in all PRCs in older developmental stages (b4b6). c1c6 Las-six3/6 A is expressed in the apical area only in young larvae (48 hpf) (c1) but can be found to be expressed in the apical PRCs and some individuals showed faint expression in the eye PRCs (c5) of older larvae. No expression was found in the posterior PRCs (c6). d1d3 Expression of Las-frizzled5/8 is limited to the apical area (d1). f1f3 Las-nk2.1 expression is limited to the apical area (Scalebars 100 μm in columns 1, 2; 50 μm in column 3; 5 μm in columns 4–6)

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