Fig. 12
Expression of Ino-Gsx during development of the cephalopod Idiosepius notoides. Dorsal (d)–ventral (v), anterior (a)–posterior (p), and left (l)–right (r) axes indicate the orientation. a Gsx is expressed in the region of the cerebral (cg) and palliovisceral ganglia (pvg) of stage 19 individuals. Inserted image depicts other optical section highlighting the mantle (m). b In addition to the optic ganglia, Ino-Gsx is expressed in the cerebral ganglia that expand anteroventrally in direction of the eyes of stage 19 individuals (arrowheads). c Optical section through the middle of the specimen shown in a and b which depicts the connection of the optic and palliovisceral ganglia. d Stage 22 individuals express Ino-Gsx in two domains of the cerebral ganglia, among others in the region of the future buccal lobes (double arrowheads). Note the unspecific signal in the intestines of this specimen (asterisk). Note unspecific staining (purple) in yolk sac (removed in d). ar arm, ey eye, mo mouth, o optic ganglion/lobe, y yolk. Scale bars 150 µm