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Fig. 6 | EvoDevo

Fig. 6

From: The ParaHox gene Gsx patterns the apical organ and central nervous system but not the foregut in scaphopod and cephalopod mollusks

Fig. 6

Expression of Aen-Gsx in early-stage trochophore larvae (14 hpf) of the scaphopod Antalis entalis. Dorsal (d)–ventral (v), anterior (a)–posterior (p), and left (l)–right (r) axes indicate the orientation. a–d, f, h, i are whole-mount preparations, while e and g are cryosections (cell membranes stained green, cellmask/cell nuclei labeled blue, Dapi). a Each two cells (arrowheads) express Aen-Gsx on both sides of the episphere. b–e Each one cell (4) expresses Aen-Gsx bilaterally to the anus. f Four Aen-Gsx-expressing cells (arrowheads) are located in episphere. g Aen-Gsx-expressing cells on the left side of the episphere (both cells on the right side are on another cryosection) and close to the anus (4). h, i BBA cleared specimen highlighting the position of mouth (mo), anus, and digestive tract (red stippled line) in relation to Aen-Gsx-expressing cells (arrowhead). an anus, pt prototroch. Scale bars 50 µm

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