Fig. 2

Developmental expression of ApGLNT1 in early developing embryos before symbiont transmission. Staining with ApGLNT1 antibody was performed on dissected asexual embryos within the ovarioles. All embryos are displayed with anterior of the germaria to the left. Signals representing ApGLNT1 immunoactivity, F-actin to the cell cortex, and nuclei are marked with color keys at the bottom of the figure. Confocal images of a–c show ApGLNT1 antibody staining results. Confocal images of a′-c′ show merged results from ApGLNT1 antibody, nuclei, and F-actin staining. Illustrations of a″–c″ display the embryonic characteristics corresponding to the presented developmental stages. ApGLNT1 signal in the outer layer surrounding the whole ovarioles is indicated with open arrowheads, and the signal in the inner layer surrounding the developing oocyte and embryos is indicated with closed arrowhead. a, a′, a″ Germaria and developing oocyte (stage 1 of development). a, a′ ApGLNT1 signals are detected in the membrane of follicular epithelium (closed and open arrowheads) and in the cytoplasm of the oocyte (arrow). b, b′, b″ Embryo undergoing synchronous, syncytial nuclear division with eight nuclei (stage 3 of development). b, b′ ApGLNT1 signals are localized in the membrane of follicular epithelium (closed and open arrowheads) and in the cytoplasm of the syncytial embryos (arrow). c, c′, c″ Embryo with newly segregated germ cells (stage 6 of development). c, c′ The membrane signals in the follicular epithelium remain detectable in the periphery of the embryos (closed and open arrowheads). Scale bars: 20 μm. cs central syncytium, dn dividing nuclei, fc follicle cells, gc germ cells, gm germarium, nc nurse cells, on oocyte nucleus, oo oocyte, ps posterior syncytium, st stage