Fig. 4

Developmental expression of ApGLNT1 in the embryos when bacteriocyte undergoes cellularization. Signals representing ApGLNT1 immunoactivity, F-actin to the cell cortex, and nuclei are marked with color keys at the bottom of the figure. All embryos are displayed with anterior of the germaria to the left. Confocal images of a–c; d–f show ApGLNT1 antibody staining results. Confocal images of a′–c′; d′–f′ show merged results from ApGLNT1 antibody, nuclei, and F-actin staining. d–f and d′–f′ are the insets shown in a–c and a′–c′, respectively. Illustrations of g–i display the embryonic characteristics corresponding to the presented developmental stages. ApGLNT1 signal in the outer layer surrounding the whole ovarioles is indicated with an open arrowhead, the inner layer surrounding the embryos is indicated with a closed arrowhead, and when two layer signals are indiscernible indicated with double arrowheads. ApGLNT1 signal located within the bacteria region is indicated with arrows. a, a′, d, d′, g Twisting embryo (stage 12 of development). a, a′, d, d′ ApGLNT1 signals are detected in the membranes of follicular epithelium (closed and open arrowheads) and in the periphery of cellularized bacteriocytes (arrow). b, b′, e, e′, h Embryo undergoing limb bud formation (stage 13 of development). b, b′, e, e′ Follicular epithelium signal can be detected (double arrowhead). ApGLNT1 signal in the periphery of the cellularized bacteriocytes becomes more condensed (arrow). c, c′, f, f′, i Extension of the germband (stage 14 of development). c, c′, f, f′ The follicular epithelium signal remains detectable in the periphery of the egg chamber (double arrowhead). ApGLNT1 signal in the periphery of the cellularized bacteriocytes becomes more restricted (arrows). Scale bars: 20 μm. bc bacteriocyte, fc follicle cells, gc germ cells, hd head, lb labial segment, mn mandible segment, mx maxilla segment, st stage, t1–t3 the three thoracic segments