Fig. 5

Developmental expression of ApGLNT1 in post-katatrepsis embryos when bacteriome undergoes maturation. Signals representing ApGLNT1 and ApVas immunoactivity, F-actin, and nuclei are marked with color keys below the figure. All embryos are displayed with anterior of the germaria to the left. Confocal images of a and b show ApGLNT1 antibody staining results. a′ and b′ show merged results from ApGLNT1 antibody, nuclei, and F-actin staining. b and b′ are the insets of a and a′, respectively. c and c′ are the negative controls. Illustrations of d and e display the embryonic characteristics corresponding to the presented developmental stages. f ApVas-positive control of staining. a, a′, b, b′ Embryo after flip (stage 16). a, a′, b, b′ The ApGLNT1 signal can be detected in the follicular epithelium (double arrowhead), in the presumptive bacterial sheath cells (arrow) of the bacteriome, and in the central nervous system (double arrow). An ApGLNT1-positive signal can be detected in the space of germband and limb bud (open arrow). c, c′ Embryo undergoes retraction (stage 17). c Embryo stained with only secondary antibody and merged with the F-actin staining and c′ merged with nucleus staining. In the absence of anti-ApGLNT1 antibodies, no immunostaining signal is detected. f Stage 18 embryo stained with ApVas antibody as a positive control. Signal specifically appears in the germ cells. Scale bars: 20 μm. bc bacteriocyte, cns central nervous system, fc follicle cells, g gut, gc germ cells, hd head, lg legs, st stage, − Ctl: negative control, + Ctl: positive control