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Fig. 13 | EvoDevo

Fig. 13

From: Developmental basis of phenotypic integration in two Lake Malawi cichlids

Fig. 13

Size differences in skeletal derivatives of the gill arches between CA and DC larvae. ag Dissected and flat-mounted alizarin red-/alcian blue-stained gill arch skeletons of 5 dpf (a12), 8 dpf (b12) and 15 dpf (c12) CA and DC larvae. Scale bar 200 μm. d, e Mean and distribution of CB1 length (d) and surface area (e) in 15 dpf CA and DC (n = 5, each). f1g2 Dissected and flat-mounted alizarin red-/alcian blue-stained CB1 and gill raker buds in 8 dpf (f12) and 15 dpf (g12) CA and DC larvae. Scale bar 50 μm. h, i Mean and distribution of CB1 gill raker number (h) and spacing (i) in 15 dpf CA and DC (n = 5, each). j1l2 Dissected and flat-mounted alizarin red-/alcian blue-stained upper pharyngeal tooth plate in 5 dpf (j12), 8 dpf (k12) and 15 dpf (l12) CA and DC larvae. m Mean and distribution of UTP surface area in 15 dpf CA and DC (n = 5, each). n1p2 Dissected and flat-mounted alizarin red-/alcian blue-stained lower pharyngeal tooth plate in 5 dpf (n12), 8 dpf (o12) and 15 dpf (p12) CA and DC larvae (n = 5, each). Scale bar 50 μm. Q, R Mean and distribution of LTP surface area (q) and arms angle (r) in 15 dpf CA and DC (n = 5, each). ***p < 0.001, Tukey’s HSD test. Bone nomenclature after [64]. BB basibranchial, CB ceratobranchial, EB epibranchial, HB hypobranchial, PHB pharyngobranchial

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