Fig. 6

Immunolocalisation of Yorkie and EdU labelling of proliferative nuclei in the C. hemisphaerica tentacle bulb. a, b Confocal longitudinal sections of the whole tentacle bulb and base of tentacle, at two different focal levels (tangential in b and deeper in a), showing anti-Yki (red), Dapi (blue) and EdU (green) staining. Refer to Fig. 1c for interpretation of these pictures. endo endoderm. White asterisks in a, b indicate capsules of undifferentiated nematoblasts (see text and Additional file 10); white arrows point to differentiated anti-Yki-stained capsules; selected nuclei positive for Yki are indicated by white arrowheads. c–f higher magnification view of the area boxed in green in (b), showing Dapi (c), EdU (d) and anti-Yki (e) staining. f Combination of the three signals (anti-Yki in red, Dapi in blue and EdU in green). g, j higher magnification view of the area boxed in red in (b), showing Dapi (g), EdU (h) and anti-Yki (i) staining; (j) combination of the three signals (anti-Yki in red, Dapi in blue, EdU in green). The structure visible in i and coloured in red in j is a mature nematocyst. k Graph of fluorescence intensities for Dapi, EdU and anti-Yki (see colour legend on the right), showing their quantified variation based on the picture in f along the orange arrow (distance in abscisse is from the proximal extremity of the arrow). l Graph of fluorescence intensities for Dapi, EdU and anti-Yki (see colour legend on the left), showing their quantified variation based on the picture in j along the yellow arrow (distance in abscisse is from the proximal extremity of the arrow). Scale bars a, b 20 µm, c, f 5 µm, g, j 10 µm