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Fig.Ā 7 | EvoDevo

Fig.Ā 7

From: Comparative study of Hippo pathway genes in cellular conveyor belts of a ctenophore and a cnidarian

Fig.Ā 7

Immunolocalisation of Yorkie and EdU labelling of proliferative nuclei in the C. hemisphaerica exumbrellar epidermis. a Partial view of Dapi-stained medusa; focus on the exumbrellar epidermis. b EdU staining (following 2Ā h pulse) in the same medusa. cir can circular endodermal canal; tt bu tentacle bulb; z cen central region of the exumbrellar epidermis; z per peripheral region of the exumbrellar epidermis. The asterisk in b corresponds to an artefact. cā€“e Detailed view of the central area of the exumbrellar epidermis (z cen in a, b; blue in Fig.Ā 8a) with anti-Yki (red in c, e), EdU (green in c, e) and Dapi (white in d; blue in e) staining. fā€“i Higher magnification view of the area boxed with dotted line in (e), showing Dapi (f), EdU (g) and anti-Yki (h) staining; i combination of the three signals (anti-Yki in red, Dapi in blue and EdU in green). jā€“l Detailed view of the peripheral area of the exumbrellar epidermis (z per in a, b; red in Fig.Ā 8b) with anti-Yki (red in j, l), EdU (green in j, l) and Dapi (white in k; blue in l) staining. mā€“p Higher magnification view of the area boxed with dotted line in (l), showing Dapi (m), EdU (n) and anti-Yki (o) staining; (p) combination of the three signals (anti-Yki in red, Dapi in blue and EdU in green). q Graph of fluorescence intensities for Dapi, EdU and anti-Yki (see colour legend on the right), showing their quantified variation based on the picture in i along the yellow arrow (distance in abscisse is from the proximal extremity of the arrow). r Graph of fluorescence intensity for Dapi, EdU and anti-Yki (see colour legend on the left), showing their quantified variation based on the picture in p along the yellow arrow (distance in abscisse is from the proximal extremity of the arrow). Scale bars a, b 50Ā Āµm, c, h 20Ā Āµm, i, p 10Ā Āµm

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