Fig. 4
From: The ontogeny of choanocyte chambers during metamorphosis in the demosponge Amphimedon queenslandica
Evidence of non-clonal choanocyte chambers visualized by differential CM-DiI labeling of postlarval and juvenile choanocyte chambers. Nuclei are stained with DAPI (blue), and cells derived from the larval epithelium are labeled with CM-DiI (red). a A choanocyte chamber completely labeled with CM-DiI. This chamber could have formed by a single progenitor cell (clonal) or multiple CM-DiI-labeled progenitor cells (non-clonal). b A non-clonal choanocyte chamber indicated by a large cluster of CM-DiI-labeled (arrowhead) and unlabeled choanocytes in a single chamber. c Two small clusters of CM-DiI-labeled choanocytes (arrowheads) in a largely unlabeled chamber. The arrow shows a neighboring archeocyte also labeled with CM-DiI. (D) Unlabeled choanocyte chambers (circled by dotted line) and a single choanocyte in the extracellular matrix (arrowhead). CM-DiI-labeled vesicles are visible within the unlabeled choanocytes. Scale bars a, b, 5 μm; c, d 10 μm