Fig. 7
From: The ontogeny of choanocyte chambers during metamorphosis in the demosponge Amphimedon queenslandica
Cells in early choanocyte chambers labeled with EdU. Nuclei are stained with DAPI (blue) and EdU (green), and cilia are immunofluorescently labeled with an anti-acetylated alpha tubulin antibody (red). a A confocal section of a 36 hpr postlarva pulse labeled with EdU from 30 to 36 hpr. Clusters of EdU-positive nuclei are observed. b A high-magnification image of clusters of EdU-positive cells (putative early choanocyte chambers (circled with dotted line) in a 36 hpr postlarva). At this stage, the cilia (arrowheads) are observed inside early choanocyte chambers. Scale bars a 20 μm; b 10 μm