Fig. 3
Expression of Pdu-dpp during caudal regeneration of Platynereis dumerilii (16 dpa). In situ hybridizations, anterior is to the left in (a–e). a Regenerated posterior end, ventral view. The red line marks the site of amputation. Regenerated tissues are to the right. The last mature segment is on the left side. b No expression of Pdu-dpp in the pygidium, posterior growth zone region and anal cirri (ventral view). c Pdu-dpp is expressed dorsally in the notopodium at the segment boundary (black arrowheads) and in the lower notopodium (white arrowheads). d Ventral expression in the trunk region near the segment boundary (black arrows). e Pdu-dpp expression in cell clusters on the dorsal side of the youngest segments. f–h Optical transverse sections of segments at different stages of maturation with focus on parapodia, ventral side down. Expression domains indicated in (c–e) are labeled accordingly (ac) anal cirri, (dc) dorsal cirrus, (nep) neuropodium, (nop) notopodium, (pb) parapodial bud, (pp) parapodia, (sg) segmental boundary (vc) ventral cirrus. Scale bar equals 200 µm