Fig. 5
Expression of Pdu-Meis/hth during caudal regeneration of Platynereis dumerilii (16 dpa). In situ hybridizations, anterior is to the left for (a–e). a Regenerated posterior end, ventral view. The red line marks the site of amputation. Regenerated tissues are to the right. The last mature segment is on the left side. Strong Pdu-Meis/hth expression in the coelothel of the forming and maturing segments and subepidermal cells of the posterior growth zone region (blue staining in the trunk region). b, c Expression in the anal cirri is limited to 4–6 cells near the base of the cirri (white arrows) Ventral view. d, e Broad Pdu-Meis/hth expression in the notopodium. Expression in a few cells in the center of the dorsal cirrus (black brackets). f–h Optical transverse sections of segments in different stages of maturation with focus on parapodia, ventral side down. g, h show close ups of notopodia. (ac) anal cirrus, (dc) dorsal cirrus, (nep) neuropodium, (nop) notopodium, (pb) parapodial bud, (pp) parapodia, (vc) ventral cirrus. Scale bar equals 200 µm