Fig. 8From: Temporally and spatially dynamic germ cell niches in Botryllus schlosseri revealed by expression of a TGF-beta family ligand and vasa Germ and follicle progenitor cells form two main clusters in stage C1 secondary buds. a Ventral view of a three-dimensional reconstruction of a secondary bud containing vasa-positive germ cells (red) and tgfβ-f-positive follicle progenitors (green) at stage C1. Nuclei are visualized with DAPI (blue). b Optical sagittal section of the secondary bud shown in a along the white dotted line. Two GFCs are visible, one dorsal and one ventralBack to article page