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Fig. 4 | EvoDevo

Fig. 4

From: Neuronal fate specification by the Dbx1 transcription factor is linked to the evolutionary acquisition of a novel functional domain

Fig. 4

Transcriptional activity of Dbx1 domains. a Experimental design of the luciferase assay allowing to assess the transcriptional activity of Gal4-fused domains of mouse Dbx1. b, c Quantification of the luciferase signal in the absence (b) or presence (c) of Groucho. Each dot represents one well of transfected cells. Values were normalized to 1 in control (Gal4-only); the dashed line marks the baseline of luciferase expression. VP16 and Engrailed repressor domain (EnR) fused to Nkx6.1 HD were used as positive and negative controls, respectively. The means are depicted by a horizontal bar, red indicates a significant difference compared to control (p < 0.01 using both Kolmogorov–Smirnov and Mann–Whitney tests), and green indicates no significant difference. Both RDs behave as Groucho-dependent transcriptional repressors, whereas the Cter domain has no transcriptional activity in this assay

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EvoDevo

ISSN: 2041-9139