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Fig. 5 | EvoDevo

Fig. 5

From: Fibronectin contributes to notochord intercalation in the invertebrate chordate, Ciona intestinalis

Fig. 5

Ci-Fn knockdown specifically blocks the ability of medial protrusions to drive productive notochord intercalation. a, b′′′ Representative micrographs showing sections of phalloidin-stained (blue) late tailbud embryos co-transfected as labeled. Red and boxes (a, b) indicate location of zoomed in images (a′, b′′′). a′, b Representative section displaying typical medial lateral polarity of phalloidin enriched protrusions in non-intercalating cells. a′′, b′′ Representative section displaying typical medial localization of nuclei in non-intercalating cells. c, j′ Still images from movies of embryos co-transfected as labeled. cf′ Representative embryo with bilateral incorporation of Brac>GFP showing invagination of notochord cells in 4-um orthogonal sections (white outlines, c′–f′) taken from Supplemental Movie 1. Dashed lines in (cf) indicate location of orthogonal images (c′–f′). gj′ Representative embryo with unilateral incorporation of Brac>GFP showing medial protrusions of notochord cells (arrows), taken from Supplemental Movie 5. Images represent 10- or 20-min intervals, time stamp in upper right corner. Scale bars in μm. Embryos oriented anterior to the left

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