Fig. 2

Expression pattern of paired class genes Dg-gsc (a–c), Dg-hbn (d–f), Dg-otp (g–i), Dg-otx (j–l) and Dg-pax6 (m–o) in adult females of Dinophilus gyrociliatus. The expression pattern of the respective gene is shown in ventral (a, d, g, j, m) and lateral (ventral side to the left, b, e, h, k, n) views as well as in schematic drawings in ventral view (c, f, i, l, o). Dg-gsc is expressed in the posteroventral region of the brain, the anterior pharyngeal epithelium and two small cell populations close to the second prostomial ciliary band (a–c). Dg-hbn shows a spot-like expression, though the individual patches are linked to form a continuous band in the anterior region and two separate posteroventral spots (d–f). Dg-otp (g–i) and Dg-otx (j–l) are also expressed in the anterior and posterior region of the brain: Dg-otp shows a narrow region of expression in the anterior region of the brain (g–i), while the posterior spots are more clearly demarcated in Dg-otx (j–l). Dg-pax6 is found to not only pattern the area of the eyes, but also extend further along the brain surface as well as into the brain (m–o). br brain, cbg ciliary band gland, ey eye, fg frontal gland, mo mouth opening, np neuropil, pcb prostomial ciliary band, phb pharyngeal bulb, vrcc ventral root of the circumesophageal connective