Fig. 7
Expression pattern of Dg-foxG, Dg-nk2.1, Dg-otp and Dg-pax6 in adult females of Dinophilus gyrociliatus, which are labeling a probable dorsal sensory region including innervation of eyes and sensory cilia (Dg-hbn and Dg-otx are included in the schematic drawings, based on information shown in previous plates). The assumed overlap of the expression domains is shown in a schematic drawing in dorsal (a), lateral (ventral side to the left, b) and ventral views (c), with similar color coding than employed in the other plates (Dg-foxg—yellow, Dg-hbn—red, Dg-nk2.1—turquoise, Dg-otp—dark purple, Dg-otx—pink, Dg-pax6—dark green). Reflective microscopy was used to correlate the NBT/BCIP-precipitation pattern (shown in d–k in cyan) with acetylated α-tubulin-like immunoreactivity (acetylated α-tubulin-LIR, pink) and DAPI-labeled nuclei (yellow). d Sagittal section of the lateral region of the brain and e sagittal section of the median region of the brain with Dg-foxg expression domains, f horizontal section of the ventral region of the brain and g sagittal section through the median region of the brain with Dg-nk2.1 expression domains, h horizontal section of the ventral region of the brain and i sagittal section through the brain with Dg-otp expression domains, j horizontal section through the dorsal region of the brain and k oblique section through the dorsal region of the brain with Dg-pax6 expression domains. br brain, cb ciliary band, eso esophagus, mo mouth opening, no nuchal organ, np neuropil, pcb prostomial ciliary bands, pcc prostomial compound cilia, phb pharyngeal bulb, vcf ventral ciliary field