Fig. 3

Gonad regeneration by morphallaxis. a Summary of amputation scheme and regeneration results for gonad regeneration. Worms were double-amputated (black vertical lines), removing the posterior end and 7 anterior segments. Anterior segments removed included the gonadal segments, segments 6 and 7. Worms regenerated only four head segments anteriorly by epimorphosis but gonads regenerated by morphallaxis in the segments that were previously segments 9 and 10, now segments 6 and 7. Dark gray regenerated segments; Green gonadal segments. Note the posterior shift in segment identity (numbers) after amputation. b Unamputated control worm with gonads revealed by PRIle-piwi1 expression. c Double-amputated worm at 5 dpa, with PRIle-piwi1 expression in gonads (testes) of the segment that was formerly segment 9 (a non-gonadal segment) but is now positioned as segment 6. t testis, o ovary, s stomach