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Fig. 2 | EvoDevo

Fig. 2

From: Conservation and divergence of bHLH genes in the calcisponge Sycon ciliatum

Fig. 2

Heatmap representation of expression profiles of Sycon ciliatum bHLH genes identified in this study. The expression levels were calculated as described in the methods quantitative analysis section (expected_count from RSEM package normalized between datasets with DESeq package) and then log 10 transformed. The colour scale is from blue (lowest) through white (medium) to red (highest). In each section of the heatmap – ‘embryonic development’, ‘metamorphosis’, and ‘adult body axis’ – the stage at which the gene has the highest expression level and at the same time the expression level is statistically significantly (padj ≤ 0.1) higher than at the reference stage was marked with the ‘+’ symbol. The reference stage for ‘embryonic development’ and ‘adult body axis’ is the ‘non.reproductive middle’, for the ‘metamorphosis’ it is the ‘swimming larvae’. The genes expressed at the statistically significantly higher level in ‘embryonic development’, ‘metamorphosis’, and ‘adult body axis’ than at the reference stage were marked with ‘*’ at the heatmap’s right margin. The stages with underlined names are illustrated. Note that early vitellogenesis to late postinversion samples are mid-body slices, composed of somatic tissues and oocytes or embryos

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