Fig. 3

Expression atlas of AAAP-536 and APC-8904 genes in asexual A. pisum during bacteriocyte formation. Staged embryos hybridized with DIG-labeled antisense (a–j) and sense riboprobes (k, l) of AAAP-536 and APC-8904. Signals were revealed by NBT/BCIP brown precipitates. All embryos are displayed with anterior of the germaria to the left. The cephalic lob (cl) and head (hd) of germband are indicated in the posterior of the egg chamber to the right. Arrowheads mark relatively strong AAAP-536-positive signals in a, b. Dashed lines indicate the location of germ cells. Arrows indicate AAAP-536 signals detected in the follicle cells in c–e. a–e S-shaped embryo (stage 11), twisting embryo (stage 12), limb bud formation (stages 13–14), and flip embryo (stage 15); transcripts of AAAP-536 were detected in the bacterial region at all stages shown. Insets in a, b are magnifications of the relatively strong signals of AAAP-536 (arrowheads). f–j Expression of APC-8904 was detected in the bacterial region. k, l Stage 13 embryos hybridized with sense riboprobes. No signal was detected with AAAP-536 sense riboprobes, but ubiquitous signals were detected with APC-8904 sense riboprobes. m Illustration displaying presented developmental stages. In situ signals of AAAP-536 and APC-8904, location of germ cells, and location of Buchnera are indicated by the color key. Scale bars are all 20 μm. bc Buchnera symbionts, cl cephalic lobe, gc germ cells, hd head, st stage, t1–3 thoracic segments