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Fig. 4 | EvoDevo

Fig. 4

From: Amino acid transporters implicated in endocytosis of Buchnera during symbiont transmission in the pea aphid

Fig. 4

Expression atlas of AAAP-536 and APC-8904 genes in asexual A. pisum during bacteriome maturation. Staged embryos hybridized with DIG-labeled antisense (ad, fi) and sense riboprobes (e, j) of AAAP-536 and APC-8904. Signals were revealed by NBT/BCIP brown precipitates. All embryos are displayed with anterior of the germaria to the left. Dashed lines indicate the location of germ cells. Arrows indicate signals detected in the follicle cells and yellow arrowheads mark non-specific signals. ad Post-flip embryo (stage 16), germband retraction (stages 17–18), and muscle formation (stage 19); transcripts of AAAP-536 were detected in the bacterial region at all developmental stages. fi Expression of APC-8904 was detected in the bacterial region. e, j Stage 18 embryos hybridized with sense riboprobes. Non-specific signals of AAAP-536 were detected in the follicle cells (arrows) and the terminal region of the rostrum (yellow arrowhead) [74]. Ubiquitous signals were detected with APC-8904 sense riboprobes. k Illustration displaying presented developmental stages. In situ signals of AAAP-536 and APC-8904, location of germ cells, Buchnera, and gut are indicated by the color key. Scale bars are all 20 μm. bc Buchnera symbionts, cns central nervous system, g gut, gc germ cells, hd head, lg legs, st stage

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