Fig. 5

Relative expression of transporter genes in developing embryos before stage 5 and after stage 6. Comparison of AAAP-536 and APC-8904 expression between embryos before stage 5 and after stage 6 of development. a Outline of dissection. Set A: germaria, oocytes (st 0–2) and embryos at or before stage 5 of development; set B: embryos at or older than stage 6 of development. Set A and set B samples were separated by discriminating morphological recognizable germ cells (gc) and also the significantly enlarged follicle cells (arrowhead) in stage 6 embryos. b Results of quantitative reverse transcription real-time PCR. Expression of AAAP-536 and APC-8904 in aphid tissues normalized against three reference genes (GAPDH, EF1α and RPL32). Bar heights indicate mean of the gene expressions across three biological replicates, and error bars indicate 95% confidence interval estimates of the mean expression. Student t test comparisons of gene expression levels between embryos before stage 5 and embryos after stage 6 are not significant (p value = 0.26 in AAAP-536 and p value = 0.32 in APC-8904). gm germaria, gc germ cells, st stage