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Fig. 7 | EvoDevo

Fig. 7

From: The neuromuscular system of Pycnophyes kielensis (Kinorhyncha: Allomalorhagida) investigated by confocal laser scanning microscopy

Fig. 7

Serotonergic nervous system of Pycnophyes kielensis. Maximum projection micrographs obtained with a confocal laser scanning microscope. The nervous system was stained with primary antibodies against serotonin and appears red. Cell nuclei where stained with DAPI and appear blue together with autofluorescence of the cuticle. In d autofluorescence of the cuticle appears green. a brain of P. kielensis with neuropil, flask-shaped somata anterior and posterior of the neuropil, and the ventral nerve cord. b 3D reconstruction of a showing the dense distribution of nuclei anterior and posterior of the neuropil. c Same 3D reconstruction as in b but turned 90° horizontally to show the neuropil ring as seen from posterior. d Overview of the nervous system with neuropil ring of the brain and ventral nerve cord running ventrally toward the anterior, where it attaches at the ventral nerve cord attachment site, and toward the posterior with a ganglion in the center of segment six behind the pharyngeal bulb. e Detail of the neuropil and ventral nerve cord that, when the introvert is inverted, runs from the neuropil toward the anterior and then posteriorly with a ganglion in segment six. ga ganglion, lan lateral nerve cord, np neuropil, so somata, vnc ventral nerve cord, vnca ventral nerve cord attachment site

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