Fig. 8

Gene expression in emu versus chicken limb buds; Patched-1, Gli3 and HoxD13. a Expression of Patched-1 mRNA in emu limb buds, at stages 21 and 24. Localization is shown by in situ hybridization; levels are shown by RNA-seq (with chicken as control). A lower level of Patched-1 expression was observed in the emu forelimb bud. b Expression of Gli3 mRNA expression in emu forelimb buds (stage 21). Localization is shown by in situ hybridization; levels are shown by RNA-seq (with chicken as control). Gli3 expression was elevated in emu forelimb bud versus the hindlimb bud at stages 20–21. c Expression of HoxD13 in expression in emu forelimb buds (stage 21). Localization is shown by in situ hybridization; levels are shown by RNA-seq (with chicken as control). HoxD13 expression was lower in emu forelimb bud versus the hindlimb bud