Rfam database annotation of the maternally packaged sncRNA transcriptome of A. limnaeus.
a The most abundant sncRNAs are 16–17 nucleotides in length and annotate as antisense RNAs, while the second most abundant group are sequences that are 26 nucleotides in length that annotate as ribosomal RNA (see panel c for a color key to annotation category). b The highest diversity of unique sncRNA sequences is in the 15 and 17 nucleotide length categories. Note the enrichment of miRNA sequences in the 20–22 nucleotide range as expected, even though miRNAs are not a dominant part of the sncRNA transcriptome. c Some annotation categories have distinct size ranges, while others span the entire range of sizes explored in this study. d Putative micro-RNA precursor structures and consensus mature sequences (highlighted in yellow) annotated as mir-181a (Alim-mir-181a1-3) and mir-10b (Alim-mir-10b1-4)