Fig. 1

Sequence comparison of Drosophila Robo3 and Tribolium Robo2/3. a Schematic comparison of the two receptors showing conserved domain structure and percent identity between individual ectodomain elements. The highest degree of sequence conservation occurs within the Slit-binding Ig1 domain (70% identity). While both proteins share the evolutionarily conserved CC0 and CC1 motifs, the TcRobo2/3 cytodomain (206 aa) is less than half the length of the Robo3 cytodomain (452 aa). b Protein sequence alignment. Structural features are indicated below the sequence. Fn domains have been re-annotated relative to Evans and Bashaw [11] based on revised predictions of beta strand locations. Identical residues are shaded black; similar residues are shaded gray. Ig immunoglobulin-like domain, Fn fibronectin type III repeat, Tm transmembrane helix, CC conserved cytoplasmic motif