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Table 1 Survival, fertility, and transmission rates for CRISPR replacement of robo3

From: CRISPR-based gene replacement reveals evolutionarily conserved axon guidance functions of Drosophila Robo3 and Tribolium Robo2/3

Injection line

# embryos injected

% (no.) adult G0 survival

% (no.) fertile G0 adults

% (no.) G0 founders

% (no.) HDR F1 progeny

nos-Cas9.P (BDSC #54591)

300

31 (93/300)

66 (61/93)

25 (5/20)

43 (5/5, 5/10, 2/10, 1/5, 2/5)

  1. robo3 TcRobo2/3 donor and gRNA plasmids were coinjected into nos-Cas9 embryos. Injected individuals (G0) were crossed as adults to Sco/CyOwg, and the resulting F1 progeny were screened by PCR for the presence of the expected robo3 TcRobo2/3 allele. Although 61 G0 crosses produced F1 progeny, only 20 of these were screened. The percentage of crosses producing at least one modified allele (founders) is indicated along with the frequency of modified F1 recovered from each founder (average recovery was 43%, range 20–100%). BDSC, Bloomington Drosophila Stock Center; HDR, homology-directed repair
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EvoDevo

ISSN: 2041-9139